Protocol for Polyclonal Antibody Production in Sheep
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Day 0 Physical examination, preimmune serum sample-100
mls
Day
14 First immunization- 1-75 mg protein in Complete Freunds Adjuvant, s.c.
multiple sites 0.5 ml/site: total of 10ml
Day
28 Second immunization -1-75 mg protein in Incomplete Freunds Adjuvant,
as above
Day
38 Serum Test Sample- 25-30 mls
Day
56 Third immunization- 1-75 mg protein in Incomplete Freunds Adjuvant,
as above
Day
66 Production bleed: 250 mls
Day
80 Fourth immunization- as above
Day
90 Production bleed: 250 mls
Day
118 Fifth immunization-as above
Day 128 Final production bleed: 500 mls
Sheep
(Suffolk)
Age........................... >1year
Weight...................... approximately 100-125 lbs
Gender...................... female
Quarantine........................... 7 to 14 days
Injection volume.................. 10 ml
4 month schedule, total of 1L serum: $875