Product #: PTX-002 anti-Fx1A
Sheep
Anti-Fx1A
For the Induction of Accelerated Passive Heymann
(membranous) Nephritis
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Materials supplied:
Sheep
immunoglobulin, 10 ml, 20 mg/ml
Freund's complete adjuvant, 10 ml
Plastic syringe 20 ml capacity, plugged end
Anti-Fx1A antibody, 25 ml for the induction of membranous nephritis in
25 rats.
Materials needed:
Approved
Institutional Animal Care and Use Committee (IACUC) protocol
Gloves
Bucket of ice
Brinkman homogenizer or equivalent (must have at least a 6" probe
and be capable of speeds of 15,000 rpm)
2 Plastic syringes, 10 ml
3 Glass syringes. 5 ml capacity
21 ga needles
25 Male Sprague-Dawley rats (175-200 gm)
Anesthetic: (consult your IACUC veterinarian)